Evolution of aflatoxin gene cluster

Blogging on Peer-Reviewed ResearchIgnazio Carbone and colleagues published a recent analysis of the evolution of the aflatoxin gene cluster in five Aspergillus fungi entitled “Gene duplication, modularity and adaptation in the evolution of the aflatoxin gene cluster” in BMC Evolutionary Biology. The authors were able to identify seven modules pairs of genes whose history of duplication were highly correlated. Several genomes of Aspergillus have been sequenced along with more Eurotioales fungi.In the Carbone et al manuscript, Figure 2 shows the pattern of duplication among members o the cluster with panel A showing the relative number of duplicates of each gene and the colors representing the species containing the duplicates. I would be interested in an even more systematic study of duplication patterns in the Eurotiales and Aspergilli as several classes of genes like sugar transporters and enzymes related to saprobic growth and plant matter degradation are also expanded. I expect that this pattern of duplication is unexpectedly high, but wonder how broadly it can be seen across the clade.The authors use panel B to explore the correlation of duplications so for example, in purple, the aflT/aflQ gene pairs are closest in the dendrogram indicating their duplication pattern is highly correlated even though they are not physically adjacent. It would be helpful to have a better description of substrates that the individual enzymes can utilize if there is some relationship between the compounds for genes with correlated duplication history. Certainly the order and specificity/generality of enzymes needed in a pathway correlates with patterns of molecular evolution as seen in Anthocyanin biosynthesis for example and recent unpublished work presented at SMBE2007 from Dave Des Marais showed that patterns of selection on duplicated genes is indicative of subfunctionalization.Figure 2 from manuscriptFigure 3 shown below, shows the gene order reconstruction in several of the Aspergillus genomes studied in attempt to identify the overall clustering of these genes across the lineages. The authors conclude from this reconstruction that reorganization to bring these genes together happened more recently stating:

We hypothesize that gene modules that are contiguous in one species and noncontiguous in others are the result of rearrangements in an ancestral species. 

This sort of rearrangement of a cluster to bring together genes has been seen such as the DAL cluster in Saccharomyces.I don’t know how much further research has been done to identify other putative clusters through comparative analyses. Certainly this was looked at in recent comparative genome paper but I don’t recall any major identification of new biosynthesis clusters based on unexpected synteny or clustering of PKS or NRPS genes.Figure 3 from manuscript

One thought on “Evolution of aflatoxin gene cluster”

  1. Hi…
    for gene clustering from where did you took the sequence???Any specific tool or software used???Also what are the criteria you fixed for chossing the sequence….please let me know as I have to do that

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