Next gen sequencing technology

Nature has an overview of what goes in and out of next generation sequencers with an interview with a smiling Chad Nusbaum from the Broad Institute. Most of these have been out and about for a while, but it seems that the hayride/bandwagon is starting to pick up more steam as GT‘s Genome Scan has several posts about sequencing referencing J. Craig V, George Church, and the Nature news article (not free).

Note that Solexa is no longer the cool name – “Genome analyzer” being the name for the machine that was previously called Solexa 1G. I’m holding out hope for funnier names in the future. I do feel that ABI’s choice of SOLiD is more exciting than 310/3700/3730 that is as inspiring as HAL9000.

But I mean if your technology is called pyrosequencing, I am hoping Roche will come up with a firey or at least smoldering play on words if they rename 454 again (GS FLX for now).

One thought on “Next gen sequencing technology”

  1. Roche doesn’t own the rights to Pyrosequencing chemistry, Qiagen does. Qiagen recently purchased a company called Biotage, which is the company that owned the rights to the chemistry. Pyrosequencing has nothing to do with temperature, it has to do with measuring Pyrophosphate (ppi) whenever dNTPs are incorporated in the sequencing reaction. It is a real time, quantitative approach to sequencing. Roche works with the genome wide applications, Qiagen targets everything else.

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