First release of N.tetrasperma and N.discreta

The JGI in collaboration with our lab at Berkeley have released the Neurospora tetrasperma (mat A) and N. discreta (mat A) genome sequences and annotation after about two years of work.  These are two closely related species to the well studied laboratory workhorse Neurospora crassa.

The N.tetrasperma assembly (8X) has an N50 of 976kb and is highly colinear with the N.crassa genome.  With the JGI, we’ve also done some additional 454 sequencing which will represent an improved assembly and 23X coverage in the next release.  We also did some comparative scaffolding and can basically double that N50 – most of which looks good when compared to the improved V2 assembly.

The N.discreta assembly (8X) is also quite good with an N50 of 2.3 Mb. For comparison, the V7 of N.crassa has an N50 of 664 kb. although with genetic map information the 250+ contigs can be scaffolded into 7 chromosomes with 146 unmapped contigs.

Both N.discreta and N.tetrasperma genomes contain about 10k predicted genes similar to counts in other related species like N.crassa and Podospora anserina.

We’re finalizing several analyses to present at the Asilomar meeting to describe these Neurospora genomes and comparisons with other Sordariomycete species.

5 thoughts on “First release of N.tetrasperma and N.discreta”

  1. Awesome! I am very excited to see the results, especially with my favorite N. tetrasperma. Anyone sequencing Agaricus bisporus to see if there are commonalities for pseudohomothallism?

  2. Thanks Dan. Fixed the link and the image. We have a 2nd assembly which is in chromosomes, we’re waiting for some QA and migration of the annotation before it goes out.

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