Research area:Xiaorong Lin lab focuses on key infectious mechanisms of the model human fungal pathogen Cryptococcus neoformans. We investigate cryptococcal life cycle and morphogenesis, and how these processes affect the disease progression in animal models. We are also interested in examining the role of cryptococcal mannoproteins in host-pathogen interactions and the molecular mechanisms underpinning cryptococcal adaptation to host conditions. Furthermore, we are working with other groups at UGA in the development of novel antifungal treatments.
Applicant qualification: Highly motivated candidates with a Ph.D. degree and strong skills in genetics, biochemistry, molecular biology, or cell biology are encouraged to apply. Experience with mammalian cell culture, animal models of microbial diseases, or immunology is a plus. Qualified candidates are expected to work independently as well as in an interactive and dynamic team environment, with good written and oral communication skills.
Application: Applicants should send (1) their CV with bibliography, (2) a cover letter with a statement of research interests and career goals, and (3) contact information for three references to Dr. Xiaorong Lin by email: Xiaorong.Lin@uga.edu.
Postdoc positions in the Heitman lab and through the Molecular Mycology Training Program at Duke University, NC State, and University of North Carolina are available as of December 1, 2014.
The Heitman lab at Duke University is seeking Postdoctoral Fellow applicants. The lab focuses on molecular determinants of development and virulence in the pathogenic basidiomycetes Cryptococcus neoformans and Cryptococcus gattii with complementary studies in both model and pathogenic fungi including Cryptococcus amylolentus, Filobasidiella depauperata, Cryptococcus heveanensis and other related basidiomycetes including Kwoniella and Malassezia species, species from the Candida pathogenic complex (C. albicans, C. lusitaniae), and the zygomycete Mucor circinelloides.
Areas of research interest include:
modes and impact of unisexual and sexual reproduction
structure, function, and evolution of the mating type locus
calcineurin in fungal virulence and as a novel antifungal drug target
comparative fungal genomics of a species cluster of human fungal pathogens
molecular networks that orchestrate infection of the host and development
RNAi based pathways operating during mitotic and sexual development and epimutational gene silencing
mechanisms of action of natural products and antifungal drugs
Applications should send pdfs of curriculum vitae, reprints/preprints, a statement of research accomplishments and interests, and letters of recommendation to Joseph Heitman at firstname.lastname@example.org.
An article in PLoS Pathogens by Morris et al describe a hypothesis about the evolution and origins of plant pathogens applying the parallel theories to the emergence of medically relevant pathogens. The authors highlight the importance of understanding the evolution of organisms in the context of emerging pathogens like Puccinia Ug99 for our ability to design strategies to protect human health and food supplies. Both bacterial and fungal pathogens of plants are discussed but I (perhaps unsurprisingly) focus on the fungi here. Continue reading Origins and evolution of pathogens→
The Tremella mesenterica genome portal is now live at the JGI. The genome is ~28Mb and the JGI annotation group predicted 8,313 genes, a significantly larger number of peptides predicted for C. neoformans (~7000; 18Mb genome) which may represent new and interesting genes or aspects of gene loss in the Cryptococcus yeast lineage.
Tremella is a Basidiomycete jelly fungus and an interesting study system from the perspective of discovery of novel lignin degrading enzymes. It also occupies an interesting phylogenetic position being an outgroup to the human pathogenic yeast Cryptococcus neoformans and C. gattii. Comparative genomics on this system may also provide insight into the interesting evolution of the large mating-type locus that was formed through various rearrangements resulting in conversion from a tetrapolar to biopolar mating system.
Tremella may also be an important source of understanding wood degradation and how it differs in jelly fungi from the more distantly related Agaricomycotina (mushroom forming). The fungus is reasonably easy to grow in the laboratory and also to collect from nature. It can handle some desiccation to survive during a dry period only to swell up after moisture is available. It is also called Witch’s butter and Tom Volk has a summary of its features on his FOTM page. It can often be confused with a phylogenetically distinct jelly fungus named Dacyromyces, usually the differences can be best be determined microscopically. See what kinds of Tremella people have been finding at the Mushroom Observer.
An avid reader pointed out that I was not entirely thorough in describing that we don’t enough about the V8 agar media that is used to induce mating in Cryptococcus. In fact a great deal of work on mating in this fungus had focused on identifying what pathways are induced by V8 agar that induce mating. It was shown that inositol stimulates mating through use of defined media containing inositol (Xue et al, 2007). This paper interestingly explores plant-fungal interactions and Cryptococcus suggesting that mating may occur preferentially on plants in cases where inositol is abundant.
It is also worth noting that V8 media contains a high level of copper ions and it was also pointed out to me that Jef Edman’s lab showed that melanin mutants have mating defects, and both phenotypes are suppressed by copper. And more recently (Lin et al, PLOS Genetics 2006) found that alleles of the Mac1 copper regulated transcription factor are a QTL influencing hyphal growth and melanin production, and showed that copper can enhance hyphal growth.
So the role of copper and interplay with V8 agar media and how this induces mating is actually quite known.
C XUE, Y TADA, X DONG, J HEITMAN (2007). The Human Fungal Pathogen Cryptococcus Can Complete Its Sexual Cycle during a Pathogenic Association with Plants Cell Host & Microbe, 1 (4), 263-273 DOI: 10.1016/j.chom.2007.05.005
A paper in IJSEM describes a new species in the Cryptococcus basidiomycete yeast lineage. The name is proposed as Cryptococcus keelungensis sp. nov. for a strain isolated from the sea surface microlayer. Its identity as a Cryptococcus sp was determined by sequencing of 26S rDNA D1/D2 and ITS loci and molecular phylogenetics. This is quite diverged from the human pathogen Cryptococcus neoformans and C. gattii as the new species falls in the order Filobasidiales while C. neoformans is classified in the order Tremellales. Interestingly, based on the phylogeny in the paper it seems to be relatively close to newly discovered Cryptococcus himalayensis.
C.-F. Chang, C.-F. Lee, S.-M. Liu (2008). Cryptococcus keelungensis sp. nov., an anamorphic basidiomycetous yeast isolated from the sea-surface microlayer of the north-east coast of Taiwan INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 58 (12), 2973-2976 DOI: 10.1099/ijs.0.65773-0
A new and improved annotation of Cryptococcus neoformans var grubii strain H99 (serotype A) has been made available in GenBank and the Broad Institute website. This update is collaboration between several groups providing data and analyses and the genome annotation team at the Broad Institute.
Some changes noted by the Broad Institute include:
“This release of gene predictions for the serotype A isolate Cryptococcus neoformans var. grubii H99 is based on a new genomic assembly provided by Dr. Fred Dietrich at the Duke Center for Genome Technology. The new assembly consists of 14 nuclear chromosomes and a single 21 KB mitochondrial chromosome, and has resulted in a reduction of the estimated genome size from 19.5 to 18.9 Mb. Improvements in the assembly and in our annotation process have resulted in a set of 6,967 predicted protein products, 335 fewer than the previous release.”
NY Times article on how bark beetle are spreading fungi and causing a pine tree die-off in the West. There is also a recent article on how populations of the fungi from different latitudes are adapted to different temperatures (Rice et al, Forest Pathology, 2008).